IMMOBILIZATION OF CANDIDA-CYLINDRACEA LIPASE ON METHYL ACRYLATE-DIVINYL BENZENE COPOLYMER AND ITS DERIVATIVES

Several series of porous beads were prepared by suspension polymerizat ion of methyl acrylate and divinyl benzene in the presence of one or t wo kinds of porogen. The activity of Candida cylindracea lipase (E.C.3 .1.1.3) (CCL) adsorbed on these carriers was investigated. Some rules were found for the influence of pore structure such as specific surfac e area, average pore diameter, and pore volume on the immobilization o f CCL. Functional groups attached to these carriers could increase the ir binding capacity. The optimal conditions for adsorbing CCL were stu died. PMA-30-175 and HFPMA-30-175 was chosen for the preparation of im mobilized CCL, and the effects of pH, ionic strength, and temperature on the immobilized CCL were compared with those on dissolved CCL.