AMINO-ACID-METABOLISM DURING BATCH CULTURE OF A MURINE HYBRIDOMA, AFP-27

This paper presents batch culture data of the murine hybridoma, AFP-27 , cultured in conventional basal media and in a nutrient-rich modified version. Expression of antibody was fivefold higher in the enriched f ormulation, with significant product secretion in the decline phase. C ultures were initiated at conventional inculation densities (1 similar to 2 x 10(5) viable cells ml(-1)) and high inoculation densities (1.5 similar to 1.7 x 10(6) viable cells ml(-1)). Amino acid levels have b een reported for all cultures, with apparent differences described. Re lative levels of intracellular amino acids are also reported, with sig nificant accumulation of proline, glycine and alanine. The results hav e significance in the design of enriched media which are clearly benef icial for commercial production of antibodies from hybridomas.