CATALYTIC DETERMINATION OF SULFIDE IN BLOOD

A catalytic method, based on the iodine-azide reaction, of the determi nation of sulfide in whole human blood is described. The method involv es generation of hydrogen sulfide in an evolution-absorption apparatus by addition of sulfuric acid and subsequent trapping of the generated hydrogen sulfide in sodium hydroxide solution. The catalytic method a llows the determination of sulfide without any interference from other sulfur compounds present in blood. A linear calibration graph was obt ained over the range 1 x 10(-6)-5 x 10(-4) mol dm(-3) sulfide and the proposed method is compared with an ion-selective electrode method.