FACTORS AFFECTING GROWTH AND SUSCEPTIBILITY TESTING OF HELICOBACTER-PYLORI IN LIQUID-MEDIA

In order to increase the database for in vitro growth and/or susceptib ility testing in liquid media, we evaluated the growth of Helicobacter pylori in broth media containing 5% sheep blood. We also compared the effect of bismuth on the growth of H. pylori in broth media containin g 10% fetal calf serum with the effect on growth in media containing 0 .5% starch. In contrast to the result seen with agar, we found that sh eep blood, whether whole or laked, inhibited the growth of H. pylori i n broth media. In addition, we found that bismuth inhibited growth in media with starch but that this inhibition was negated in media with s erum.