EXPRESSION OF THE HUMAN INTERLEUKIN-2 RECEPTOR-GAMMA CHAIN IN INSECT CELLS USING A BACULOVIRUS EXPRESSION VECTOR

The gene encoding the gamma-chain of the human Interleukin-2 receptor was expressed in lepidopteran insect cells using the baculovirus expre ssion vector system. The corresponding gene was inserted under the pol yhedrin promoter of the Autographa californica nuclear polyhedrosis vi rus and expressed in the Spodoptera frugiperda insect cell line Sf9 du ring viral infection. The recombinant receptor protein was identified by immunoblotting in cell lysates, prepared from insect cells infected with the recombinant virus. At 40 h post infection the corresponding protein was detected as two major bands with apparent molecular weight s of 50-60 kDa using a rabbit anti-human IL-2R gamma-receptor specific antiserum. Metabolic labelling with [S-35]-methionine and SDS-PAGE an alysis of the recombinant baculovirus infected insect cells verified t he immunoblotting data. The expressed IL-2R gamma- protein could also be determined on the surface of infected insect cells by flow cytomete r analysis.