SEQUENCE OF THE 3'-TERMINAL REGION OF THE RNA OF A MITE TRANSMITTED POTYVIRUS FROM HORDEUM-MURINUM L

The 3026 nucleotides upstream of the 3'-polyadenylated tract of a mite transmitted virus from Hordeum murinum L. were cloned and sequenced, and portions of the sequence were expressed in Escherichia coli. Seque nce comparisons with wheat streak mosaic virus (WSMV), Agropyron mosai c virus (AgMV) and Hordeum mosaic cirus (HoMV), three mite transmitted potyviruses, and potato virus Y (PVY), the type member of the genus P otyvirus, revealed that the virus is probably a potyvirus, but distinc t from WSMV, AgMV, HoMV, and PVY. Serological tests further demonstrat ed these differences and that the virus is serologically related to an other potyvirus, brome streak mosaic virus (BrSMV). We conclude that t he virus should be named as the Hordeum isolate of BrSMV.