CYTOCHEMICAL-LOCALIZATION OF ACID-PHOSPHA TASE-ACTIVITY IN HEBELOMA-CYLINDROSPORUM ISOLATED OR ASSOCIATED WITH PINUS-PINASTER

The study about Hebeloma cylindrosporum's acid phosphatase activity ac cording to the phosphorus content of the culture medium, has been real ised by using a new staining:the lanthanum. Electron energy loss spect roscopy (EELS) and electron spectroscopy imaging (ESI) have been used to verify the presence of the stain. These technicals display a hetero geneous acid phosphatase ativity on the plasmalemma. The phosphorus de ficienced mycelium presents an excreted acid phosphatase activity only in the storage section. Acid phosphatases are detected on the plasmal emma, the tonoplast, the polyphosphate granules and the lipid globuls. Whatever the phosphorus content it is, the walls are stainless.