THE STRUCTURE STUDY OF A NEW SAIKOSAPONIN

Saikosaponin O was isolated from Bupleutum smithii Wolff var. parvifol ium Shan et Y. Li. Its structure was determinated by NMR methods. From TOCSY and DQF - COSY spectra, the residues of monosaccharides and all their assignments were acquired. The anomeric configurations were obt ained by (3)J(H1H2). The HMQC - COSY spectrum wasused successfully for C-13 assignment since it was difficult to do it only by CH - COSY spe ctrum. All the glucosidic linkages were obtained from HMBC spectrum ba sed on the H-1 and C-13 assignments NOSY spectrum also gave the concor dant result about the configurations and the linkage positions.