GLUCURONIC ACID-SILICA, A NOVEL SUPPORT FOR HIGH-PERFORMANCE CATION-EXCHANGE CHROMATOGRAPHY

Authors
LEE HG JARRETT HW
Citation
Hg. Lee et Hw. Jarrett, GLUCURONIC ACID-SILICA, A NOVEL SUPPORT FOR HIGH-PERFORMANCE CATION-EXCHANGE CHROMATOGRAPHY, Journal of liquid chromatography, 18(6), 1995, pp. 1113-1122
Citations number
15
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
18
Issue
6
Year of publication
1995
Pages
1113 - 1122
Database
ISI
SICI code
0148-3919(1995)18:6<1113:GAANSF>2.0.ZU;2-M
Abstract
Glucuronic acid was covalently coupled to aminopropyl derivatized sili ca by reducing the Schiff base with NaCNBH3. This glucuronic acid-sili ca has a functional pKa in the pH 3-4 range and binds 202 mg hemoglobi n per gram material at pH 5. The support is a high-performance cation- exchanger and exhibited only minor hydrophobic interaction with applie d samples. It also gave reasonably constant retention of the proteins tested.