DETERMINATION AND ANALYSIS OF HUMAN SERUM ALPHA-1-ACID GLYCOPROTEIN BY LIQUID-CHROMATOGRAPHIC METHODS

Human serum alpha-l-acid glycoprotein (orosomucoid, AGP) with particul ar interest as a tumor marker was measured, separated, purified and an alyzed for its carbohydrate constituents. In order to demonstrate the changes in the concentration and composition of serum AGP induced by m alignant diseases an improved method of sample preparation based on so lvent extraction, ion exchange and reversed phase liquid chromatograph ic methods were applied. In preliminary studies significantly elevated serum AGP levels and increased fucose content in the carbohydrate moi ety were found in cancer patients in comparison to the healthy individ uals.