MICELLAR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF SERUM BILIRUBIN SPECIES WITH DIRECT SAMPLE INJECTION

A rapid reversed-phase high performance Liquid chromatographic method is described using a C-8 column and a mobile phase containing sodium d odecyl sulfate (SDS) as a modifier to separate the four bilirubin spec ies present in serum: unconjugated, monoconjugated, diconjugated and b iliprotein. The results show that using SDS as a mobile phase modifier improves separation efficiency and increases sample solubility. This simple HPLC procedure allows direct sample injection and makes it poss ible to quantitatively determine bilirubin species in biological fluid s.