FUNCTIONAL EXPRESSION OF THE GLUTAMATE UPTAKE SYSTEM FROM CORYNEBACTERIUM-GLUTAMICUM IN ESCHERICHIA-COLI

Glutamate uptake in the Gram-positive Corynebacterium glutamicum is me diated via a binding protein-dependent transport system, which is enco ded by the gluABCD gene cluster. Cloning of these genes in an expressi on vector and subsequent transformation of the resulting plasmid allow s different strains of the Gram-negative bacterium Escherichia coli to grow on glutamate as sole carbon and nitrogen source. However, overex pression of the glutamate uptake system results in growth inhibitory e ffects, probably due to the particular topology of the binding protein .