Sn. Shchelkunov et al., COMPARISON OF THE GENOME DNA-SEQUENCES OF BANGLADESH 1975 AND INDIA 1967 VARIOLA VIRUSES, Virus research, 36(1), 1995, pp. 107-118
The nucleotide sequences of genome DNAs and the deduced amino acid seq
uences of proteins from potential open reading frames (ORFs) of variol
a smallpox viruses from outbreaks in India in 1967 and in Bangladesh i
n 1975 have been compared and the analyses of the sequences are update
d. Alignment of the DNAs revealed 99.3% base sequence identity. Of the
200 potential encoded proteins of each virus, 122 were identical, 42
showed substitution of a single amino acid, 11 showed two residues cha
nges, and the remainder were more diverged. The variant proteins were
encoded mainly in the near-terminal regions of each genome. The most c
onserved region between the variola DNAs included ORFs A33L to A49R, w
hich is a relatively poorly conserved region compared with vaccinia vi
rus.