D. Johansson et al., EFFECTS OF ANOXIA ON ENERGY-METABOLISM IN CRUCIAN CARP BRAIN-SLICES STUDIED WITH MICROCALORIMETRY, Journal of Experimental Biology, 198(3), 1995, pp. 853-859
Crucian carp (Carassius carassius L.) is an exceptionally anoxia-toler
ant vertebrate. To determine whether isolated crucian carp brain tissu
e survives anoxia and whether it displays anoxic metabolic depression,
heat production (using microcalorimetry), lactate production, ethanol
production and the maintenance of ATP, ADP and AMP levels and energy
charge were measured in telencephalic brain slices during anoxia. In r
esponse to anoxia, heat output decreased by 37%, corresponding to a 31
% fall in ATP turnover rate. Adenylate phosphates and energy charge we
re well maintained and no ethanol was produced during anoxia, It is co
ncluded that crucian carp brain tissue has an intrinsic capacity to to
lerate anoxia and that it responds to anoxia by depressing metabolic r
ate and elevating the glycolytic rate, thereby maintaining ATP levels.