ANALYSIS OF TRIACYLGLYCEROL POSITIONAL ISOMERS BY SILVER ION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Silver ion chromatography, utilizing two commercially available HPLC c olumns connected in series, was used to separate a variety of triacylg lycerol positional isomers. The isomers had a fatty acid composition o f ABA and AAB, where A and B were fatty acids containing zero to three double bonds. Isocratic solvent systems of 0.5% to 1.0% acetonitrile in hexane were used to separate the triacylglycerol isomers. This meth odology can be used to rapidly determine the isomeric purity of synthe sized or isolated triacylglycerols and to isolate specific positional isomers.