MOLECULAR-CLONING OF A NOVEL HUMAN CDNA ON CHROMOSOME 1Q21 AND ITS MOUSE HOMOLOG ENCODING A NUCLEAR-PROTEIN WITH DNA-BINDING ABILITY

We previously reported that human chromosome 1q21 or 1q23-q24 carries a transformation suppressor gene(s) for Kirsten sarcoma virus-transfor med NIH3T3 cells. In this study, we have isolated a novel human cDNA o n 1q21 (designated as YL-1) as a candidate, then cloned its mouse homo log. Human and mouse YL-1 cDNA shared 96% in amino acid homology and p resumably encoded a transcription factor-like polypeptide. An analysis using a specific antibody revealed the nuclear localization of YL-1 p rotein. The bacterially expressed YL-1 could bind to DNA. Thus, YL-1 p rotein possibly functions as a transcriptional regulator. (C) 1995 Aca demic Press, Inc.