MOLECULAR-CLONING AND CHARACTERIZATION OF MOUSE HS1

Here we report the cloning of a cDNA and a genomic DNA encoding the mo use homolog of human HS1, a hematopoietic cell-specific protein-substr ate of nonreceptor type protein-tyrosine kinase(s). Sequence analysis of the mouse HS1 cDNA revealed that it is highly homologous to human H S1 (total percent match = 84%) especially in the amino-terminal half, which contains unique repeating motifs, and in the carboxyl-terminal S rc-homology 3 domain. As is the human counterpart, the mouse HS1 gene is expressed exclusively in hematopoietic cells. Genomic fragments cov ering most of the HS1 gene were isolated and used to map this gene to mouse chromosome 16. (C) 1995 Academic Press, Inc.