An outbreak of bacillary haemoglobinuria was recorded in 60 out of 110
sheep in Ludhiana, Punjab, India. The condition was clinically charac
terised by fever, haemoglobinuria, constipation, weakness of hind quar
ters followed by recumbency, respiratory distress and death in 16 shee
p. Haematological studies revealed moderate to severe degrees of anaem
ia associated with leucocytosis. Plasma gamma-glutamyl transferase, al
kaline phosphatase and creatinine phosphokinase activities were signif
icantly higher in haemoglobinuric sheep. Babesiosis and copper poisoni
ng were ruled out on stained blood film examination and from blood min
eral profiles, respectively. Post-mortem examination of affected sheep
revealed no gross changes. Pure cultures of Clostridium haemolyticum
isolated from heart blood, liver, kidney and spleen of freshly killed
sheep confirmed the disease. Parenteral administration of procaine pen
icillin was effective in the treatment of affected sheep.