Analysis of the nucleotide sequence of the rfbX gene of Shigella flexn
eri revealed that it contained a high proportion of rare codons, as pr
eviously observed in the analysis of the O-antigen polymerase-encoding
gene rfc [Morona et al., J. Bacteriol. 176 (1994) 733-747]. The rfbX
gene encodes a hydrophobic, 46-kDa protein, with 12 potential transmem
brane-spanning domains, that shows structural homology with gene produ
cts encoded in many rfb regions, and with Orf0416 of the rff region of
Escherichia coli K-12 which has also been identified as a member of t
his class of proteins. Attempts to clone rfbX independent of other rfb
genes, and to identify the protein product of rfbX have proven unsucc
essful. Analysis of plasmids containing various deletions within the r
fb region suggest that the 5' end of rfbX plays an indirect regulatory
role in expression of the dTDP-rhamnose biosynthetic enzymes, encoded
by rfbBCAD. We speculate that RfbX is a cytoplasmic membrane protein
which functions in the transport of the O-antigen repeat unit.