GENETIC-ANALYSIS OF THE RFBX GENE OF SHIGELLA-FLEXNERI

Citation
Df. Macpherson et al., GENETIC-ANALYSIS OF THE RFBX GENE OF SHIGELLA-FLEXNERI, Gene, 155(1), 1995, pp. 9-17
Citations number
32
Categorie Soggetti
Genetics & Heredity
Journal title
GeneACNP
ISSN journal
03781119
Volume
155
Issue
1
Year of publication
1995
Pages
9 - 17
Database
ISI
SICI code
0378-1119(1995)155:1<9:GOTRGO>2.0.ZU;2-C
Abstract
Analysis of the nucleotide sequence of the rfbX gene of Shigella flexn eri revealed that it contained a high proportion of rare codons, as pr eviously observed in the analysis of the O-antigen polymerase-encoding gene rfc [Morona et al., J. Bacteriol. 176 (1994) 733-747]. The rfbX gene encodes a hydrophobic, 46-kDa protein, with 12 potential transmem brane-spanning domains, that shows structural homology with gene produ cts encoded in many rfb regions, and with Orf0416 of the rff region of Escherichia coli K-12 which has also been identified as a member of t his class of proteins. Attempts to clone rfbX independent of other rfb genes, and to identify the protein product of rfbX have proven unsucc essful. Analysis of plasmids containing various deletions within the r fb region suggest that the 5' end of rfbX plays an indirect regulatory role in expression of the dTDP-rhamnose biosynthetic enzymes, encoded by rfbBCAD. We speculate that RfbX is a cytoplasmic membrane protein which functions in the transport of the O-antigen repeat unit.