A DNA fragment from Pseudomonas aeruginosa containing the rpoH gene en
coding the heat-shock sigma factor sigma(32) has been cloned and seque
nced. The gene is expressed in Escherichia coli and complements an rpo
H(-) strain. An open reading frame encoding 284 amino acids shows 61%
identity and 78% similarity to the RpoH protein of Escherichia coli.