PARTIAL-PURIFICATION AND CHARACTERIZATION OF BFI57I AND BFI89I, RESTRICTION ENDONUCLEASES FROM DIFFERENT STRAINS OF BUTYRIVIBRIO-FIBRISOLVENS

Authors
MOHN WW TEATHER RM
Citation
Ww. Mohn et Rm. Teather, PARTIAL-PURIFICATION AND CHARACTERIZATION OF BFI57I AND BFI89I, RESTRICTION ENDONUCLEASES FROM DIFFERENT STRAINS OF BUTYRIVIBRIO-FIBRISOLVENS, Gene, 155(1), 1995, pp. 131-132
Citations number
4
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
155
Issue
1
Year of publication
1995
Pages
131 - 132
Database
ISI
SICI code
0378-1119(1995)155:1<131:PACOBA>2.0.ZU;2-5
Abstract
Two class-II restriction endonucleases (ENases), Bfi57I and Bfi89I, we re partially purified from Butyrivibrio fibrisolvens OB157 and OB189, respectively. Bfi57I(isoschizomer Sau3AI) had the DNA recognition/clea vage sequence 5'-/GATC-3'; it is not inhibited by Dam methylation, but is partially inhibited by M . BamHI methylation. Bfi89I (isoschizomer EaeI) had the recognition/cleavage sequence 5'-Y/GGCCR-3'; unlike the EaeI isoschizomer it is not fully inhibited by M . HaeIII methylation .