NEONATAL TOLERANCE TO AN IMMUNODOMINANT T-CELL REACTIVITY DOES NOT CONFER RESISTANCE TO EAMG INDUCTION IN LEWIS RATS

Citation
Te. Zoda et al., NEONATAL TOLERANCE TO AN IMMUNODOMINANT T-CELL REACTIVITY DOES NOT CONFER RESISTANCE TO EAMG INDUCTION IN LEWIS RATS, Journal of neuroimmunology, 57(1-2), 1995, pp. 35-44
Citations number
36
Categorie Soggetti
Neurosciences,Immunology
Journal title
ISSN journal
01655728
Volume
57
Issue
1-2
Year of publication
1995
Pages
35 - 44
Database
ISI
SICI code
0165-5728(1995)57:1-2<35:NTTAIT>2.0.ZU;2-S
Abstract
The overall goal of this study was to determine, during induction of e xperimental autoimmune myasthenia gravis (EAMG) in Lewis rats, the rel ative importance of acetylcholine receptor (AChR)-reactive helper T ce lls associated with one particular immunodominant fine specificity. Th us, experiments presented below were designed to evaluate the immunopa thological role played by helper T cells with reactivity against the A ChR alpha subunit region associated with amino acid residues 100-116 ( i.e., alpha 100-116); in particular, the relationship between T cell r eactivity with this specificity and disease induction was assessed. In order to examine the importance of this T cell reactivity, Lewis rat neonates were made T cell tolerant to a synthetic peptide alpha 100-11 6 and subsequently evaluated for anti-AChR antibody production and res ulting neuromuscular dysfunction. Results indicated that although T ce ll reactivity against the alpha 100-116 peptide could be effectively r emoved from the Lewis T cell repertoire, tolerized Lewis rats immunize d with AChR could undergo an active anti-AChR antibody response that p roduced symptoms of EAMG. Thus, other AChR T cell reactivities appeare d capable of providing adequate help to B cells leading to production of anti-AChR antibodies with pathogenic potential.