C. Hery et al., ADHESION TO HUMAN NEURONS AND ASTROCYTES OF MONOCYTES - THE ROLE OF INTERACTION OF CR3 AND ICAM-1 AND MODULATION BY CYTOKINES, Journal of neuroimmunology, 57(1-2), 1995, pp. 101-109
Monocytes but not unstimulated lymphocytes adhered to human neurons an
d astrocytes in primary culture, as demonstrated by double labeling. T
he expression of VCAM-1 was higher on neurons than on astrocytes, wher
eas that of beta 1, alpha 1, alpha 2, alpha 4 and alpha 5 chains from
the integrins and of ICAM-1 was identical on both types of cells. The
expression on neurons of ICAM-1, but not of integrins, was up-regulate
d by exogenous tumor necrosis factor (TNF) alpha, interleukin (IL)-1 a
lpha and interferon (IFN)-gamma. The same was observed on astrocytes a
ssociated with a sharp increase in the expression of VCAM-1. Adhesion
between monocytes and neurons or astrocytes was 80% inhibited by mAbs
directed against the CR3 determinant on monocytes or against ICAM-1 on
neural cells but not by any of the other mAbs against adhesion protei
ns that were tested. Finally, the level of endogenous production of IL
-1 alpha and TNF alpha was greatly increased after the adhesion of mon
ocytes to CNS cells.