DIRECT EMBRYO FORMATION FROM EPIDERMAL-CELLS OF CARROT HYPOCOTYLS

Authors
MASUDA H OOHASHI SI TOKUJI Y MIZUE Y
Citation
H. Masuda et al., DIRECT EMBRYO FORMATION FROM EPIDERMAL-CELLS OF CARROT HYPOCOTYLS, Journal of plant physiology, 145(4), 1995, pp. 531-534
Citations number
16
Categorie Soggetti
Plant Sciences
Journal title
Journal of plant physiology → ACNP
ISSN journal
01761617
Volume
145
Issue
4
Year of publication
1995
Pages
531 - 534
Database
ISI
SICI code
0176-1617(1995)145:4<531:DEFFEO>2.0.ZU;2-C
Abstract
A novel embryo-forming system has been developed in which carrot somat ic embryogenesis takes place in an one-stage process from epidermal ce lls in the absence of 2,4-D, provided hypocotyl explants are incubated in a liquid MS medium containing 2,4-D for 12-24 h. In this system, s omatic embryos develop directly via the formation of cluster cells fro m epidermal cells without intervening callus and a suspension-culture phase.