Ecs. Chan et al., EVIDENCE FOR 2 DISTINCT LOCOMOTORY PHENOTYPES OF TREPONEMA-DENTICOLA ATCC-35405, Oral microbiology and immunology, 10(2), 1995, pp. 122-124
Citations number
5
Categorie Soggetti
Immunology,Microbiology,"Dentistry,Oral Surgery & Medicine
Using a two-layer system, a bottom layer containing treponema cells su
spended in NOS (New Oral Spirochete)-Noble agar medium or NOS-Bacto ag
ar medium and overlaid with cell-free NOS-agarose medium resulted in t
he spirochete cells migrating into the top layer. However, if the posi
tions of the medium layers were reversed with the cells inoculated int
o the bottom layer containing NOS-agarose, there was no migration into
the upper layer. This suggests migration of the spirochetes away from
Bacto and Noble agars. Using a 3-layer system in which cells were ino
culated into a middle layer consisting of NOS-agarose medium and sandw
iched between cell-free NOS-agarose medium layers, cells remained with
in the middle layer. If the cells were inoculated into a middle layer
consisting of NOS-Bacto agar medium while the upper and lower layers r
emained unchanged, cells migrated into both upper and lower layers. If
cells that had migrated into the upper layer were transferred into a
middle layer, they virtually all migrated into the upper layer repeate
dly. Cells that had migrated into the lower layer and transferred to t
he middle layer migrated repeatedly into the lower layer. These result
s suggest the possible existence of two distinct locomotory phenotypes
within this strain of treponeme.