Yh. Kim et al., A NEW GENE WITH SEQUENCE AND STRUCTURAL SIMILARITY TO THE GENE ENCODING HUMAN LYSYL OXIDASE, The Journal of biological chemistry, 270(13), 1995, pp. 7176-7182
We have isolated a number of recombinant clones from a human skin fibr
oblast cDNA library that contain extensive sequence homology to severa
l coding domains within the human lysyl oxidase mRNA. Using one of the
se lysyl oxidase-like cDNAs, we obtained several overlapping genomic D
NA recombinants. Restriction mapping and DNA sequence analysis reveale
d that the complete sequence of the lysyl oxidase-like mRNA was encode
d by seven exons distributed throughout 25 kilobases of genomic DNA. E
xons 2-6 encoded the region of greatest homology to lysyl oxidase. The
size of these five exons, moreover, was exactly the same as the size
of the corresponding exons within the lysyl oxidase gene. Northern blo
t analysis also revealed the concomitant appearance of lysyl oxidase a
nd lysyl oxidase-like mRNA in several human tissues. It appears theref
ore that the genes encoding lysyl oxidase and a lysyl oxidase-like pro
tein share a common evolutionary origin and may also be functionally r
elated.