IDENTIFICATION AND CHARACTERIZATION OF PROTEIN-KINASE CKII ISOFORMS IN HELA-CELLS - ISOFORM-SPECIFIC DIFFERENCES IN RATES OF ASSEMBLY FROM CATALYTIC AND REGULATORY SUBUNITS

Protein kinase CKII (formerly casein kinase II) can be isolated as a h eterotetramer, containing two catalytic (a or alpha') and two regulato ry (beta) subunits. We have characterized the forms of CKII in HeLa ce lls using antibodies specific for the alpha or alpha' subunits. Follow ing metabolic labeling with [S-35]methionine, whole cell soluble extra cts were analyzed by immunoprecipitation and gel electrophoresis. Both alpha and alpha' coprecipitate with beta and with each other. However , when extracts are depleted of alpha, a pool of CKII containing only alpha' and beta is identified. Similarly, depletion of alpha' revealed a pool exclusively of alpha and beta. Therefore, we propose that ther e are three distinct isoforms of CKII within HeLa cells with different catalytic subunit stoichiometries (alpha(2) beta(2), alpha alpha'beta (2), and alpha'(2) beta(2)). With our immunodepletion procedure we hav e characterized the isoforms by activity analysis, turnover of pulse-l abeled subunits, and by localization in subcellular fractions obtained from labeled cells. We have also analyzed complex formation between t he catalytic and regulatory subunits by examining the differences in t he rate of signal incorporation into subunits in immunoprecipitates ob tained from continuously labeled and pulse-labeled cells. We have foun d that the alpha(2) beta(2) and alpha alpha'beta(2) isoforms assemble relatively slowly (12-16 h), whereas complex formation of the alpha'(2 ) beta(2) isoform occurs more rapidly (2-4 h). Analysis of isoform com plex formation in subcellular fractions from pulse-labeled cells revea led that the majority of nuclear CKII is assembled in the nucleus from free catalytic and regulatory subunit polypeptides.