ENZYMATIC-PROPERTIES OF THE STONEFISH (SYNANCEIA-VERRUCOSA BLOCH AND SCHNEIDER, 1801) VENOM AND PURIFICATION OF A LETHAL, HYPOTENSIVE AND CYTOLYTIC FACTOR

Authors
GARNIER P GOUDEYPERRIERE F BRETON P DEWULF C PETEK F PERRIERE C
Citation
P. Garnier et al., ENZYMATIC-PROPERTIES OF THE STONEFISH (SYNANCEIA-VERRUCOSA BLOCH AND SCHNEIDER, 1801) VENOM AND PURIFICATION OF A LETHAL, HYPOTENSIVE AND CYTOLYTIC FACTOR, Toxicon, 33(2), 1995, pp. 143-155
Citations number
59
Categorie Soggetti
Toxicology,"Pharmacology & Pharmacy
Journal title
Toxicon → ACNP
ISSN journal
00410101
Volume
33
Issue
2
Year of publication
1995
Pages
143 - 155
Database
ISI
SICI code
0041-0101(1995)33:2<143:EOTS(B>2.0.ZU;2-2
Abstract
The crude venom of the stonefish (Synanceia verrucosa) possesses numer ous enzymatic properties (hyaluronidase, eight esterases and ten amino peptidases). Verrucotoxin was isolated by DEAE and hydroxyapatite chro matography, followed by FPLC gel filtration on Superdex 200 HR 10/30. It was found to be a glycoprotein with a mel. wt of 322,000 +/- 2000, comprising four subunits, 2 alpha (83,000) and 2 beta (78,000). Verruc otoxin, as the crude venom, is lethal for mice, haemolyses washed rabb it erythrocytes and induces a fall in arterial pressure in anaesthetiz ed rats.