RESISTANCE OF MORPHOLINO PHOSPHORODIAMIDATE OLIGOMERS TO ENZYMATIC DEGRADATION

Authors
HUDZIAK RM BAROFSKY E BAROFSKY DF WELLER DL HUANG SB WELLER DD
Citation
Rm. Hudziak et al., RESISTANCE OF MORPHOLINO PHOSPHORODIAMIDATE OLIGOMERS TO ENZYMATIC DEGRADATION, ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 6(4), 1996, pp. 267-272
Citations number
13
Categorie Soggetti
Medicine, Research & Experimental","Biothechnology & Applied Migrobiology
Journal title
ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT → ACNP
ISSN journal
10872906
Volume
6
Issue
4
Year of publication
1996
Pages
267 - 272
Database
ISI
SICI code
1087-2906(1996)6:4<267:ROMPOT>2.0.ZU;2-4
Abstract
Oligomers possessing the Morpholino phosphorodiamidate backbone were e valuated for resistance to a variety of enzymes and biologic fluids, A 25-mer was incubated with nucleases, proteases, esterases, and serum, and the reaction mixtures were directly analyzed by MALDI-TOF mass sp ectrometry, The 25-mer was completely resistant to 13 different hydrol ases and serum and plasma. The excellent resistance of the Morpholino phosphorodiamidates to enzymatic attack indicates their suitability fo r in vivo use.