SSE8647I, A NEW TYPE-II RESTRICTION-ENDONUCLEASE FROM A STREPTOMYCES SPECIES CUTTING AT 5'-AG GWCCT-3'/

Authors
ISHINO Y NOMURA Y KATO I
Citation
Y. Ishino et al., SSE8647I, A NEW TYPE-II RESTRICTION-ENDONUCLEASE FROM A STREPTOMYCES SPECIES CUTTING AT 5'-AG GWCCT-3'/, Nucleic acids research, 23(5), 1995, pp. 742-744
Citations number
9
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
23
Issue
5
Year of publication
1995
Pages
742 - 744
Database
ISI
SICI code
0305-1048(1995)23:5<742:SANTRF>2.0.ZU;2-O
Abstract
We isolated and characterized a new type II restriction endonuclease w hich recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence fro m a microorganism belonging to Streptomyces species. This enzyme cleav es adenovirus 2 DNA at eight sites, but does not cleave lambda phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and phi X174 DNAs.