NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF THE SACCHAROMYCES-CEREVISIAE RPL19A GENE ENCODING A HOMOLOG OF THE MAMMALIAN RIBOSOMAL-PROTEIN L19

A gene designated RPL19A has been identified in the region downstream from the 3'-end of the Saccharomyces cerevisiae MIS1 gene encoding the mitochondrial C-1-tetrahydrofolate synthase. The gene codes for the y east ribosomal protein YL19 which exhibits 57.5% identity with the mam malian ribosomal protein L19. RPL19A is one of two functional copies o f the YL19 gene located on chromosome II. The disruption of RPL19A has no effect on the growth of the yeast. The RPLIPA gene contains an int ron located near the 5'-end. The 5'-flanking region contains one simil ar and one complete UAS(rgp) upstream activating sequence. RPL19A was also found to be adjacent to the chromosome II AAC3 gene, encoding the mitochondrial ADP/ATP carrier protein. The nucleotide sequence(s) rep orted in this paper has been submitted to the GenBank(tm)/EMBL data ba nk with the accession number Z36751.