Gs. Brouns et al., ASSEMBLY AND INTRACELLULAR-TRANSPORT OF THE HUMAN B-CELL ANTIGEN RECEPTOR COMPLEX, International immunology, 7(3), 1995, pp. 359-368
The B cell antigen receptor (BCR) complex consists of transmembrane (m
) lg, in non-covalent association with a disulphide-linked heterodimer
of mb-1 and B29 gene products, The MB-1-B29 heterodimer is required f
or deposition of the BCR at the plasma membrane, as well as for coupli
ng of the antigen receptor to intracellular signal transduction cascad
es, We have performed biosynthetic labelling studies using the mature
B cell line Ramos to investigate the process of assembly of the BCR co
mponents; We conclude that association of the four components, Ig-heav
y chain (HC) and -light chain (LC), MB-1 and B29, is required and suff
icient to permit exit of the BCR complex out of the endoplasmic reticu
lum (ER), With the short pulse labelling procedures used, no evidence
was found for transient participation of other molecules in complex fo
rmation, A 32 kDa glycoprotein was identified, which is serologically
related to MB-1, but has a more acidic isoelectric point (pl) and a pr
otein backbone of 21 kDa, as compared with 25 kDa for MB-1, This prote
in did not appear to participate in BCR complex formation and is most
likely degraded prior to reaching the cis-Golgi, The MB-1 component wa
s found to be the rate-limiting step in BCR complex formation, while I
g-HC, -LC and B29 are synthesized in excess. Ig-HC and -LC form disulp
hide-linked tetrameric complexes within 3 min after biosynthesis, with
which B29 and MB-1 components associate independently, followed by di
sulphide bond formation between these heterodimeric partners: While pa
rtial BCR complexes containing B29 and mlg-H(2)L(2) tetramers are rapi
dly formed and have a half-life of a few hours in the ER, entry of MB-
1 into these complexes controls exit out of this compartment.