F. Marchetti et Jb. Mailhes, VARIATION OF MOUSE OOCYTE SENSITIVITY TO GRISEOFULVIN-INDUCED ANEUPLOIDY DURING THE 2ND MEIOTIC DIVISION, Mutagenesis, 10(2), 1995, pp. 113-121
The effects of griseofulvin (GF) treatment during the second meiotic d
ivision of oogenesis were investigated by cytogenetic analysis of mous
e one-cell (1-Cl) zygotes, After determining the duration of fertiliza
tion and the second meiotic division, 1500 mg/kg GF were administered
to superovulated mice at 10, 12 or 14 h after human chorionic gonadotr
ophin (HCG) injection, The results showed that GF reduced the frequenc
ies of fertilized oocytes (P < 0.01) and of 1-Cl zygotes that reached
the first mitotic metaphase stage (P < 0.001), Aneuploidy was induced
regardless of the moment of treatment, but the various treatment times
were associated with statistically different (P < 0.05) levels of hyp
erdiploidy, The maximum frequency of hyperdiploidy (39.6%) occurred wh
en GF was given 10 h after HCG, Polyploid 1-Cl zygotes were significan
tly induced only at the 10 and 12 h treatment times and their level ne
ver exceeded 1.8% of fertilized oocytes. GF also induced abnormalities
of chromosome condensation along with centromeric chromosome associat
ions, These results support the conclusion that GF treatment during th
e second meiotic division induced aneuploidy, polyploidy, and reduced
rates of fertilization and zygotic development. Also, the time of chem
ical treatment influenced the frequencies of these effects.