IMMUNOLOCALIZATION OF PROTEIN PHOSPHATASE INHIBITOR-1 IN THE CEREBRAL-CORTEX OF THE RAT, CAT AND FERRET

Citation
Pr. Lowenstein et al., IMMUNOLOCALIZATION OF PROTEIN PHOSPHATASE INHIBITOR-1 IN THE CEREBRAL-CORTEX OF THE RAT, CAT AND FERRET, Brain research, 676(1), 1995, pp. 80-92
Citations number
38
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
00068993
Volume
676
Issue
1
Year of publication
1995
Pages
80 - 92
Database
ISI
SICI code
0006-8993(1995)676:1<80:IOPPII>2.0.ZU;2-3
Abstract
The distribution of inhibitor-1 was analysed in the neocortex of cat, ferret and rat by immunocytochemistry (at the light and electron micro scope levels) and by immunoblotting using an affinity purified antibod y which recognises both the phosphorylated and dephosphorylated forms of the protein. In each mammalian cortex immunocytochemical techniques identified inhibitor-1 predominantly in infragranular pyramidal neuro ns and, at a lower concentration, in supragranular pyramidal neurons o f cortical layers II-III, and V-VI. Within the cortical layers, neuron al cell bodies and apical dendrites were stained strongly but no immun oreactivity was associated with dendritic spines. Regional differences in intensity of staining were revealed when appropriate antibody conc entrations were used; the concentration of inhibitor-1 appeared to fol low a gradient with the highest levels in layer VI and the lowest in l ayer I. The results were confirmed by immunoblotting of microdissected cortical regions which identified the inhibitor-1 protein unambiguous ly. The distribution of inhibitor-1 is different from that reported by other investigators.