Av. Usatov et al., RELATIONSHIP BETWEEN PLASTID MUTAGENESIS INDUCED BY N-NITROSOMETHYLUREA AND AGE OF GERMINATING SUNFLOWER SEEDS AT THE TIME OF TREATMENT, Genetika, 31(2), 1995, pp. 222-227
The optimal time range for induction of plastid chlorophyll mutations
by N-nitrosomethylurea (NMU) during sunflower seed germination was det
ermined. It is shown that treatment of seeds germinating for 0 - 24 h
with NMU at concentrations 0.01% and 0.015% induces mutations only in
the range of 9 - 18 h of germination with a pronounced maximum at 12 t
o 15 h. In three-hour intervals adjacent to this maximum, a substantia
l decrease in the efficiency of induction is observed, especially duri
ng 15 to 18 h of germination.