ANALYSIS OF ENZYME-KINETIC MEASUREMENTS FOR AN ORGANIC-MEDIUM BIOCATALYST

Authors
BURTON SG DUNCAN JR
Citation
Sg. Burton et Jr. Duncan, ANALYSIS OF ENZYME-KINETIC MEASUREMENTS FOR AN ORGANIC-MEDIUM BIOCATALYST, Biotechnology techniques, 9(1), 1995, pp. 7-12
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
1
Year of publication
1995
Pages
7 - 12
Database
ISI
SICI code
0951-208X(1995)9:1<7:AOEMFA>2.0.ZU;2-Z
Abstract
Enzyme kinetic data for oxidation of phenolic substrates in an organic -medium polyphenol oxidase biocatalytic system, were analysed by techn iques relating specifically to nbn-aqueous biocatalysis. The results w ere related to steric, hydrophobic, and electronic character in the su bstrates; steric and hydrophobic character were found to influence the kinetics. Molecular modelling and kinetic results together gave an es timate of steric capacity in the binding site in the organic medium.