J. Parrado et al., A CONVENIENT PROCEDURE FOR QUANTITATIVE-ANALYSIS OF STAINED OR IMMUNOSTAINED BLOTTING MEMBRANES BY DENSITOMETRY, Biotechnology techniques, 9(1), 1995, pp. 29-30
A convenient method for analysing the information provided by blotting
membranes is described. The membranes are first air dried at room tem
perature, copied to transparency film and the intensity of the bands o
n the transparency is determined by densitometry The results show a li
near correlation between the amount of protein present and the final s
canner response.