HIGH-FREQUENCY TRANSFORMATION OF ALCALIGENES-EUTROPHUS PRODUCING POLY-BETA-HYDROXYBUTYRIC ACID BY ELECTROPORATION

Authors
PARK HC LIM KJ PARK JS LEE YH HUH TL
Citation
Hc. Park et al., HIGH-FREQUENCY TRANSFORMATION OF ALCALIGENES-EUTROPHUS PRODUCING POLY-BETA-HYDROXYBUTYRIC ACID BY ELECTROPORATION, Biotechnology techniques, 9(1), 1995, pp. 31-34
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
1
Year of publication
1995
Pages
31 - 34
Database
ISI
SICI code
0951-208X(1995)9:1<31:HTOAPP>2.0.ZU;2-K
Abstract
A strain of Alcaligenes eutrophus producing poly-beta-hydroxybutyric a cid was successfully transformed by the electroporation. The plasmid u sed was a broad host range plasmid pKT230 conferring kanamycin resista nce. The optimum yield of transformant was 0.8x10(2)/mu g DNA when 50 mu l competent cells at 10(10)/ml were pulsed by 11.5 kV/cm for 5 ms w ith 1 mu g DNA. Plasmid DNA in the A. eutrophus transformant was stabl y maintained as a monomeric structure.