STABILIZATION OF TRYPSIN BY MODIFICATION WITH COMB-SHAPED COPOLYMERS OF POLY(ETHYLENE GLYCOL) DERIVATIVE AND MALEIC-ANHYDRIDE

Authors
HIROTO M YAMADA M UENO T YASUKOHCHI T MATSUSHIMA A KODERA Y INADA YJ
Citation
M. Hiroto et al., STABILIZATION OF TRYPSIN BY MODIFICATION WITH COMB-SHAPED COPOLYMERS OF POLY(ETHYLENE GLYCOL) DERIVATIVE AND MALEIC-ANHYDRIDE, Biotechnology techniques, 9(2), 1995, pp. 105-110
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
2
Year of publication
1995
Pages
105 - 110
Database
ISI
SICI code
0951-208X(1995)9:2<105:SOTBMW>2.0.ZU;2-#
Abstract
Trypsin from bovine pancreas was coupled with copolymers of poly(ethyl ene glycol) derivative and maleic anhydride with the molecular weights of 13 kDa and 100 kDa (activated PM(13) and PM(100)). The modified tr ypsins were more stable towards autolysis and heat- or urea-treatment than nonmodified trypsin. Stabilization of trypsin caused by the chemi cal modification with activated PMs is discussed in relation to the pr otein conformation.