ENZYMATIC METHOD TO DETERMINE BIOMASS IN SKIM MILK WHEY PERMEATE MEDIUM

Authors
CHOI H LALEYE L AMANTEA GF SIMARD RE
Citation
H. Choi et al., ENZYMATIC METHOD TO DETERMINE BIOMASS IN SKIM MILK WHEY PERMEATE MEDIUM, Biotechnology techniques, 9(2), 1995, pp. 139-144
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
2
Year of publication
1995
Pages
139 - 144
Database
ISI
SICI code
0951-208X(1995)9:2<139:EMTDBI>2.0.ZU;2-G
Abstract
In this study, a new technique for biomass determination was developed . Proteases were used to separate cells from the culture broth in skim milk whey permeate medium and the solubilization of casein was achiev ed 99% by elastase, 95% by pepsin and 90% by trypsin. Elastase was fou nd to be efficient and reproducible for determining biomass in skim mi lk whey permeate medium.