EFFECTS OF PEPTONE ON HYBRIDOMA GROWTH AND MONOCLONAL-ANTIBODY FORMATION

Hybridoma WuT3 secreting a monoclonal antibody against T lymphocytes w as grown in RPMI 1640 medium supplemented with 1% human serum. The eff ect of the concentration of peptone, as an additive, was investigated on cell growth, monoclonal antibody formation, and cell metabolism ove r 0-10 g l(-1) range. It was found that 1-5 g l(-1) peptone can signif icantly promote the growth of cells and increase the formation of mono clonal antibody, especially at 3-5 g l(-1), when both the accumulating level and secretion rate of monclonal antibody are higher than that a t other peptone concentrations. Based on glucose, lactate and ammonia analysis data, the efficiency of glycolysis was assessed and the utili zation of amino acids was more efficient at 3-5 g l(-1) peptone. The c ell growth and monoclonal antibody formation were inhibited at higher peptone concentrations, e.g. 10 g l(-1).