Purpose. To measure quantitatively the change in the coaxial fundus re
flex with varying degrees of ocular misalignment. Methods. The coaxial
fundus reflex was imaged with a charge coupled device camera under co
nditions of simulated ocular misalignment ranging from 0 degrees to 7
degrees of fixation eccentricity. The effects of refractive error and
pupil size were controlled. Average gray scale brightness values were
calculated for each bright pupil image after some image processing was
performed on the raw images. Results. A reliable, sharply delineated,
minimum brightness at foveal fixation was observed. Conclusions. It i
s estimated that this technique can be automated to detect the presenc
e of 2 degrees to 3 degrees of ocular misalignment based on the differ
ence in brightness of the bright pupil images between the two eyes.