NON-CLATHRIN-COAT PROTEIN-ALPHA IS A CONSERVED SUBUNIT OF COATOMER AND IN SACCHAROMYCES-CEREVISIAE IS ESSENTIAL FOR GROWTH

Authors
GERICH B ORCI L TSCHOCHNER H LOTTSPEICH F RAVAZZOLA M AMHERDT M WIELAND F HARTER C
Citation
B. Gerich et al., NON-CLATHRIN-COAT PROTEIN-ALPHA IS A CONSERVED SUBUNIT OF COATOMER AND IN SACCHAROMYCES-CEREVISIAE IS ESSENTIAL FOR GROWTH, Proceedings of the National Academy of Sciences of the United Statesof America, 92(8), 1995, pp. 3229-3233
Citations number
40
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
92
Issue
8
Year of publication
1995
Pages
3229 - 3233
Database
ISI
SICI code
0027-8424(1995)92:8<3229:NPIACS>2.0.ZU;2-Z
Abstract
To complete the molecular characterization of coatomer, the preformed cytosolic complex that is involved in the formation of biosynthetic tr ansport vesicles, we have cloned and characterized the gene for non cl athrin-coat protein alpha (alpha-COP) from Saccharomyces cerevisiae. T he derived protein, molecular weight of 135,500, contains four WD-40 r epeated motifs (Trp/Asp-containing motifs of approximate to 40 amino a cids). Disruption of the yeast alpha-COP gene is lethal. Comparison of the DNA-derived primary structure with peptides from bovine alpha-COP shows a striking homology. alpha-COP is localized to coated transport vesicles and coated buds of Golgi membranes derived from CHO cells.