M. Ito et al., REGULATION OF BLOOD-PRESSURE BY THE TYPE 1A ANGIOTENSIN-II RECEPTOR GENE, Proceedings of the National Academy of Sciences of the United Statesof America, 92(8), 1995, pp. 3521-3525
The renin-angiotensin system plays a critical role in sodium and fluid
homeostasis. Genetic or acquired alterations in the expression of com
ponents of this system are strongly implicated in the pathogenesis of
hypertension. To specifically examine the physiological and genetic fu
nctions of the type 1A receptor for angiotensin II, we have disrupted
the mouse gene encoding this receptor in embryonic stem cells by gene
targeting. Agtr1A(-/-) mice were born in expected numbers, and the his
tomorphology of their kidneys, heart, and vasculature was normal. AT(1
) receptor-specific angiotensin II binding was not detected in the kid
neys of homozygous Agtr1A(-/-) mutant animals, and Agtr1A (+/-) hetero
zygotes exhibited a reduction in renal AT(1) receptor-specific binding
to approximate to 50% of wild-type [Agtr1A(+/+)] levels. Presser resp
onses to infused angiotensin II were virtually absent in Agtr1A(-/-) m
ice and were qualitatively altered in Agtr1A(+/-) heterozygotes. Compa
red with wild type controls, systolic blood pressure measured by tail
cuff sphygmo- manometer was reduced by 12 mmHg (1 mmHg = 133 Pa) in Ag
tr1A(+/-) mice and by 24 mmHg in Agtr1A(-/-) mice. Similar differences
in blood pressure between the groups were seen when intraarterial pre
ssures were measured by carotid cannulation. These studies demonstrate
that type 1A angiotensin II receptor function is required for vascula
r and hemodynamic responses to angiotensin II and that altered express
ion of the Agtr1A gene has marked effects on blood pressures.