VASCULARITY AND PERFUSION OF HUMAN GLIOMAS XENOGRAFTED IN THE ATHYMICNUDE-MOUSE

Citation
Hjja. Bernsen et al., VASCULARITY AND PERFUSION OF HUMAN GLIOMAS XENOGRAFTED IN THE ATHYMICNUDE-MOUSE, British Journal of Cancer, 71(4), 1995, pp. 721-726
Citations number
25
Categorie Soggetti
Oncology
Journal title
ISSN journal
00070920
Volume
71
Issue
4
Year of publication
1995
Pages
721 - 726
Database
ISI
SICI code
0007-0920(1995)71:4<721:VAPOHG>2.0.ZU;2-A
Abstract
The vascularisation and perfusion of seven subcutaneously xenografted human glioma lines established from surgical specimens has been analys ed using an anti-collagen type IV antibody to visualise the vascular w alls in combination with a perfusion marker (Hoechst 33342). A compute r-based digital image processing system was employed for quantitative analysis of the parameters. The vascular architecture of individual tu mours belonging to the same tumour line showed a consistent similarity , while substantial differences occurred between the various tumour li nes derived from different patients. Despite the presence of a large i nter-tumour variation in vascular area as a proportion of the tumour a rea, this vascular parameter clearly showed tumour line-specific chara cteristics. The perfused fraction of the tumour vessels also showed a large inter-tumour variation for all tumour lines ranging from 20% to 85%, but the majority of tumours of all lines had perfusion fractions of more than 55%. Despite large variation, the perfused vascular area as a proportion of the tumour cross-sectional area exhibited clear tum our line-specific tendencies. These observations suggest that consiste nt differences in vascular parameters are present between glioma xenog raft lines, although the tumour lines all originated from histological ly similar human high-grade gliomas. These differences may have import ant consequences for treatment and clinical behaviour of this type of tumour.