EXPRESSION OF BASIC FIBROBLAST GROWTH-FACTOR AND FIBROBLAST GROWTH-FACTOR RECEPTOR GENES IN CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS

Authors
VANNECK JW MEDINA JJ ONNEKINK C SCHWARTZ SM BLOEMERS HP
Citation
Jw. Vanneck et al., EXPRESSION OF BASIC FIBROBLAST GROWTH-FACTOR AND FIBROBLAST GROWTH-FACTOR RECEPTOR GENES IN CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS, Biochimica et biophysica acta, N. Gene structure and expression, 1261(2), 1995, pp. 210-214
Citations number
23
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1261
Issue
2
Year of publication
1995
Pages
210 - 214
Database
ISI
SICI code
0167-4781(1995)1261:2<210:EOBFGA>2.0.ZU;2-G
Abstract
Basic fibroblast growth factor (bFGF) exerts a differential effect on DNA synthesis, bFGF mRNA synthesis, and expression of FGF-receptor gen es by cultured smooth muscle cells from aortae of newborn and adult ra ts (used as a model in atherosclerosis research). Cells from adult ani mals, are more sensitive to bFGF, and bFGF triggers its own mRNA synth esis. Moreover, the level of the transcript of the FGFR-1 gene (coding for the most abundant FGF-receptor in smooth muscle cells) is higher in smooth muscle cells from adult rats. In contrast, the FGFR-3 gene o nly is expressed in smooth muscle cells from newborn rats. Crosslinkin g of [I-125]bFGF to its receptor showed 130 kDa and 160 kDa complexes both in newborn and adult smooth muscle cells.