CLEARANCE OF SENDAI VIRUS BY CD8(-CELLS REQUIRES DIRECT TARGETING TO VIRUS-INFECTED EPITHELIUM() T)

Minimal numbers of CD8(+) T cells are found in bronchoalveolar lavage (BAL) populations recovered from Sendai virus-infected mice that are h omozygous (-/-) for a beta 2-microglobulin (beta 2-m) gene disruption. The prevalence of the CD8(+) set was substantially increased in the p neumonic lungs of 8-12-week radiation chimeras made using substantiall y class I major histocompatibility complex (MHC) glycoprotein-negative beta 2-m (-/-) recipients and normal beta 2-m (+/+) bone marrow. Even so, the CD8(+) (but not the CD4(+)) lymphocyte counts were still much lower than in the (+/+)-->(+/+) controls. The (+/+)-->(+/+) and (+/+) -->(-/-) chimeras cleared Sendai virus and potent virus-immune CD8(+) cytotoxic T lymphocytes (CTL) specific for H-2K(b) + viral nucleoprote in peptide were found in the BAL from both groups. However, following irt vivo depletion of the CD4(+) population, only the (+/+)-->(+/+) mi ce were able to deal with the infection. Similarly, adoptively transfe rred, H-2K(b)-restricted CD8(+) T cells from previously-primed (+/+) m ice also failed to clear virus from the lungs of (+/+)-->(-/-) chimera s infected within 2 weeks of reconstitution with bone marrow, though t hey were effective in the (+/+)-->(+/+) controls. Sendai virus-immune CD8(+) T cells are thus unable to eliminate virus-infected beta 2-m (- /-) lung epithelial cells that might be thought to be expressing very small amounts of either isolated class I heavy chain, or class I MHC g lycoprotein that has bound beta 2-m derived from beta 2-m (+/+) T cell s or macrophages present in the pneumonic lung. Furthermore, the CD8() CTL that are being exposed to beta 2-m (+/+) stimulators in the BAL population cannot operate in some bystander mode to clear virus from r espiratory epithelium.