Cm. Baker et Hy. Wetzstein, REPETITIVE SOMATIC EMBRYOGENESIS IN PEANUT COTYLEDON CULTURES BY CONTINUAL EXPOSURE TO 2,4-D, Plant cell, tissue and organ culture, 40(3), 1995, pp. 249-254
Somatic embryos from immature cotyledons in peanut (Arachis hypogaea)
were initiated on media supplemented with 2, 4-dichlorophenoxyacetic a
cid (2, 4-D). Over 90% primary embryogenesis and 41-46% repetitive emb
ryogenesis were obtained 12 weeks after initiation by maintaining embr
yogenic cultures on medium containing 20 mg 1(-1) 2, 4-D. Maintenance
of cultures on medium with 30 or 40 mg 1(-1) 2, 4-D resulted in lower
primary and secondary embryogenesis, and proliferation of nonembryogen
ic callus. Transfer of embryogenic cultures to a secondary medium with
10 or 20 mg 1(-1) 2, 4-D significantly enhanced secondary embryogenes
is compared to basal medium without the growth regulator. The use of M
urashige and Skoog versus Finer's media had no significant effect on e
mbryogenesis (85-95%), repetitive embryogenesis (11-37%) or mean embry
o number. Secondary embryogenesis was also maintained for over one yea
r by repeated subculture of isolated somatic embryos on medium with 20
mg 1(-1) 2, 4-D.