GROUPING AND IDENTIFICATION OF LOW-TEMPERATURE BASIDIOMYCETES USING MATING, RAPD AND RFLP ANALYSES

Mating studies, and RAPD and RFLP analyses used in connection with phe netic and phylogenetic algorithms, were used to reassess the species i dentity of 23 putative strains of Coprinus psychromorbidus. The result s of the mating studies carried out among some of these strains sugges t at least four different species. Statistical analyses based on index of genetic dissimilarity and number of evolutionary steps of RAPD and RFLP data resolved 21 of the 23 putative strains into four distinct g roups. We propose that the 23 strains, previously grouped into the sin gle taxon, C. psychromorbidus, belong to four or more different taxa. Advantages of molecular characterization of fungi using the RAPD techn ique are discussed. RAPD profiles appear to be better suited to molecu lar analyses oriented toward the characterization and clustering of th e low temperature basidiomycetes than are RFLP profiles based on ribos omal DNA genes. Statistical treatment of RAPD data indicated that RAPD profiles, including all major and minor DNA bands, were highly reprod ucible.