EXPERIMENTAL-MODEL FOR LOCAL-ADMINISTRATION OF NERVE GROWTH-FACTOR INMICROSURGICAL NERVE RECONNECTIONS

An experimental model for local administration of neuronotrophic subst ances at the site of peripheral nerve lesion is presented. The model c onsists of a subcutaneously located silicone reservoir and a connectin g tube with its distal end fixed in the proximity of the severed and r epaired nerve. The results of the preliminary tests of the model are p resented, Sixty Sprague-Dawley rats were divided into two groups: cont rol (saline-treated) (n=30) and NGF-treated (n=30). After axotomy of t he sciatic nerve, an epineurial repair is performed. NGF or saline is injected daily into the subcutaneous reservoir during the first 3 week s after axotomy and a single dose in the fourth week. The regenerated nerve observed in the NGF-treated group after four weeks of treatment presents a greater percentage of myelinated axons, thicker myeline she aths, and more mature endoneurial layers, This experimental model prov ides a reliable and quantitative way to deliver neuronotrophic substan ces in site and at different administration rates. (C) 1995 Wiley-Liss , Inc.